oral administration of a seed-based bivalent rotavirus vaccine containing vp6 and nsp4 induces specific immune responses in mice
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2017
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Abstract
Rotavirus is the leading cause of severe diarrheal disease among newborns. Plant-based rotavirus vaccines have been developed in recent years and have been proven to be effective in animal models. In the present study, we report a bivalent vaccine candidate expressing rotavirus subunits VP6 and NSP4 fused with the adjuvant subunit B of E. coli heat-labile enterotoxin (LTB) in maize seeds. The RT-PCR and Western blot results showed that VP6 and LTB-NSP4 antigens were expressed and accumulated in maize seeds. The expression levels were as high as 0.35 and 0.20% of the total soluble protein for VP6 and LTB-NSP4, respectively. Oral administration of transgenic maize seeds successfully stimulated systemic and mucosal responses, with high titers of serum IgG and mucosal IgA antibodies, even after long-term storage. This study is the first to use maize seeds as efficient generators for the development of a bivalent vaccine against rotavirus.
| Reference Key |
feng2017frontiersoral
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|---|---|
| Authors | ;Hao Feng;Xin Li;Weibin Song;Mei Duan;Hong Chen;Tao Wang;Jiangli Dong |
| Journal | phytochemistry letters |
| Year | 2017 |
| DOI |
10.3389/fpls.2017.00910
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