variabilidade genética entre acessos de amendoim genetic variability among peanut accessions
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2007
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Abstract
O objetivo deste trabalho foi avaliar a variabilidade genética entre 29 acessos de amendoim (Arachis hypogaea L.), por meio de marcadores moleculares randômicos (DNA polimórfico amplificado ao acaso - RAPD). O ensaio molecular foi realizado com 31 iniciadores, dos quais 12 (39%) mostraram polimorfismo. Observou-se o total de 145 fragmentos amplificados, dos quais 35 (24%) foram polimórficos, com média de 4,67 fragmentos por iniciador e 1,13 fragmento polimórfico por iniciador. Pelo dendrograma, observou-se que os acessos foram separados em dois grupos com 89% de similaridade. Esta distribuição mostra a variabilidade existente entre os acessos das diferentes variedades botânicas, uma vez que acessos da subespécie fastigiata estão presentes nos dois grupos principais, e os acessos da subespécie hypogaea estão distribuídos pelos subgrupos A e B do grupo II do dendograma.
The objective of this study was to evaluate the genetic variability among 29 accessions of peanut (Arachis hypogaea L.) by means of random molecular markers (random amplified polimorphic DNA - RAPD). The molecular assay was performed with 31 primers, of which 12 (39%) revealed polymorphism. It was observed a total of 145 amplified fragments, of which 35 (24%) were polymorphic, with an average of 4.67 fragments by primer and 1.13 polymorphic fragment by primer. It was observed through the dendrogram that the accessions were separated into two groups with 89% of similarity. This distribution shows the variability among the accessions of the different botanical varieties, since the accessions of subspecie fastigiata are present in two principal groups, and the accessions of subspecie hypogaea are distributed in subgroups A and B from dendrogram group II.
The objective of this study was to evaluate the genetic variability among 29 accessions of peanut (Arachis hypogaea L.) by means of random molecular markers (random amplified polimorphic DNA - RAPD). The molecular assay was performed with 31 primers, of which 12 (39%) revealed polymorphism. It was observed a total of 145 amplified fragments, of which 35 (24%) were polymorphic, with an average of 4.67 fragments by primer and 1.13 polymorphic fragment by primer. It was observed through the dendrogram that the accessions were separated into two groups with 89% of similarity. This distribution shows the variability among the accessions of the different botanical varieties, since the accessions of subspecie fastigiata are present in two principal groups, and the accessions of subspecie hypogaea are distributed in subgroups A and B from dendrogram group II.
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| Authors | ;Wardsson Lustrino Borges;Gustavo Ribeiro Xavier;Norma Gouvêa Rumjanek |
| Journal | proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006 |
| Year | 2007 |
| DOI |
10.1590/S0100-204X2007000800012
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