efficient rutin and quercetin biosynthesis through flavonoids-related gene expression in fagopyrum tataricum gaertn hairy root cultures with uv-b irradiation
Clicks: 288
ID: 219379
2016
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
288 views
52 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #300 of 796 articles by views in phytochemistry letters
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 796 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Transformed hairy roots had been efficiently induced from the seedlings of Fagopyrum tataricum Gaertn. due to the infection of Agrobacterium rhizogenes. Hairy roots were able to display active elongation with high root branching in 1/2 MS medium without growth regulators. The stable introduction of rolB and aux1 genes of A. rhizogenes WT strain 15834 into F. tataricum plants was confirmed by PCR analysis. Besides, the absence of virD gene confirmed hairy root was bacteria-free. After 6 different media and different sources of concentration were tested, the culturing of TB7 hairy root line in 1/2 MS liquid medium supplemented with 30 g•l-1 sucrose for 20 days resulted in a maximal biomass accumulation (13.5 g•l-1 fresh weight, 1.78 g•l-1 dry weight) and rutin content (0.85 mg•g-1). The suspension culture of hairy roots led to a 45-fold biomass increase and a 4.11-fold rutin content increase in comparison with the suspension culture of non-transformed roots. The transformation frequency was enhanced through preculturing for 2 days followed by infection for 20 min. The UV-B stress treatment of hairy roots resulted in a striking increase of rutin and quercetin production. Furthermore, the hairy root lines of TB3，TB7 and TB28 were chosen to study the specific effects of UV-B on flavonoid accumulation and flavonoid biosynthetic gene expression by qRT-PCR. This study has demonstrated that the UV-B radiation was an effective elicitor that dramatically changed in the transcript abundance of FtPAL, FtCHI, FtCHS, FtF3H and FtFLS-1 in F. tataricum hairy roots.
| Reference Key |
ehuang2016frontiersefficient
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Xuan eHuang;Jingwen eYao;Yangyang eZhao;Dengfeng eXie;Xue eJiang;Ziqin eXu |
| Journal | phytochemistry letters |
| Year | 2016 |
| DOI |
10.3389/fpls.2016.00063
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.