detection and genotyping of hpv in women with indeterminate cytology and low-grade squamous intraepithelial lesions

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ID: 218562
2015
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Abstract
ABSTRACT Introduction: The human papillomavirus (HPV) is the main risk factor related to cervical cancer, the third most frequent type of cancer in Brazilian women. Early identification of high-risk HPV types in the normal cervix, or cervix with premalignant lesions may help prevent the progression of these lesions to cancer. Objective: This study aimed to detect and genotype HPV in women with low-grade intraepithelial lesion (LSIL) and atypical squamous cells of undetermined significance (ASC-US). Methods: Patients were selected from files of the cytology laboratory of Fundação Alfredo da Mata (FUAM), between January 2009 and July 2011, for cytological reassessment and HPV molecular detection with genotyping. Results: Out of the100 eligible patients, 70% (70/100) participated in the study; initially, 34 of them had ASC-US and 36 had LSIL. After cytological reassessment, eight (11.4%) patients showed normal cytology; 33 (47.2%), inflammatory lesions; 22 (31.4%), ASCUS; six (8.6%), LSIL; and one (1.4%), high-grade squamous intraepithelial lesions (HSIL). HPV was detected in 28.6% (20/70) of the samples. Out of the 20 HPV-positive patients, one had normal cytology, six showed inflammatory cytology, 10 showed ASCUS, two had LSIL and one, HSIL. After genotyping, the HPV types identified were: 6, 16, 58, 61, 70, 83, 84 and 85. The most prevalent HPV type was the 58. Conclusion: The presence of high-risk HPV in women with old cervical lesions, whether they have evolved or not, indicates the need to improve patient monitoring and surveillance.
ÖZET Giriş: İnsan papillomavirüsü (HPV), Brezilyalı kadınlarda en sık görülen üçüncü kanser türü olan serviks kanseriyle ilişkili başlıca risk faktörüdür. Normal servikste veya premalign lezyonlu servikste yüksek riskli HPV tiplerinin erken tespiti, bu lezyonların kansere ilerlemesini önlemeye yardımcı olabilir. Amaç: Bu çalışmada, düşük dereceli intraepitelyal lezyon (LSIL) ve önemi belirlenemeyen atipik skuamöz hücreler (ASC-US) saptanan kadınlarda HPV'nin tespit edilmesi ve genotiplendirilmesi amaçlanmıştır. Yöntemler: Hastalar, sitolojik yeniden değerlendirme ile genotipleme yoluyla HPV moleküler tespiti amacıyla Ocak 2009 ile Temmuz 2011 tarihleri arasında Alfredo da Mata Vakfı (FUAM) sitoloji laboratuvarı arşivlerinden seçilmiştir. Bulgular: Uygun kriterleri karşılayan 100 hastanın %70'i (70/100) çalışmaya katılmıştır; başlangıçta bu hastaların 34'ünde ASC-US, 36'sında ise LSIL mevcuttu. Sitolojik yeniden değerlendirmenin ardından sekiz (%11,4) hastada normal sitoloji; 33'ünde (%47,2) inflamatuar lezyonlar; 22'sinde (%31,4) ASCUS; altısında (%8,6) LSIL ve birinde (%1,4) yüksek dereceli skuamöz intraepitelyal lezyon (HSIL) saptandı. Örneklerin %28,6'sında (20/70) HPV tespit edildi. HPV pozitif 20 hastanın birinde normal sitoloji, altısında inflamatuar sitoloji, 10'unda ASCUS, ikisinde LSIL ve birinde HSIL görüldü. Genotipleme sonrasında tanımlanan HPV tipleri 6, 16, 58, 61, 70, 83, 84 ve 85 idi. En yaygın görülen HPV tipi 58'di. Sonuç: İlerleme göstermiş olsun ya da olmasın, geçmişte servikal lezyon öyküsü bulunan kadınlarda yüksek riskli HPV varlığı, hasta takibinin ve sürveyansının güçlendirilmesi gerektiğine işaret etmektedir.
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queiroz2015jornaldetection Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Francisca A. Queiroz, Danielle A. P. Rocha, Roberto Alexandre A. B. Filho, Cristina Maria B. Santos
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Journal industrial and engineering chemistry research
Year 2015
DOI
10.5935/1676-2444.20150029
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442015000300166&lng=en&tlng=en
Keywords
hpv

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