triptolide restores autophagy to alleviate diabetic renal fibrosis through the mir-141-3p/pten/akt/mtor pathway

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2017
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Abstract
Fibrosis is the major pathological feature of diabetic kidney disease (DKD). Autophagy, a process to maintain metabolic homeostasis, is obviously inhibited in DKD. Triptolide (TP) is a traditional Chinese medicine extract known for immune suppression and anti-inflammatory and anti-cancer activities. In this study, we investigated the effects of TP on autophagy and fibrosis in DKD. TP restored autophagy and alleviated fibrosis in DKD rats and high-glucose-incubated human mesangial cells. After we applied 3-methyladenine (an autophagy inhibitor) and autophagy-related gene 5-small interfering RNA (siRNA), we found that the improvement of fibrosis on TP was related to the restoration of autophagy. In addition, miR-141-3p levels were increased under high glucose but reduced after TP treatment. miR-141-3p overexpression aggravated the fibrosis and restrained the autophagy further, while miR-141-3p inhibition imitated the effects of TP. As an action target, phosphatase and tensin homolog (PTEN) showed corresponding opposite changes. After PTEN-siRNA transfection, the effects of TP on autophagy and fibrosis were inhibited. PTEN levels were downregulated, with downstream phosphorylated protein kinase B (Akt) and the mammalian target of rapamycin (mTOR) upregulated in high glucose, which were reversed by TP treatment. These findings indicate that TP alleviates fibrosis by restoring autophagy through the miR-141-3p/PTEN/Akt/mTOR pathway and is a novel therapeutic option for DKD.
Reference Key
li2017moleculartriptolide Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xiao-yu Li, Shan-shan Wang, Zhe Han, Fei Han, Yun-peng Chang, Yang Yang, Mei Xue, Bei Sun, Li-ming Chen
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Journal coordination chemistry reviews
Year 2017
DOI
10.1016/j.omtn.2017.08.011
URL
http://www.sciencedirect.com/science/article/pii/S2162253117302391
Keywords
microrna diabetic kidney disease autophagy triptolide pharmacology fibrosistherapeutics

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