thermal stability of cryptococcus sp. s-2 carboxymethyl cellulase (cscmcase) having a cellulose binding domain from a fungal exoglucanase: comparison to recombinant cscmcase

Clicks: 303
ID: 213914
2009
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #29 of 95 articles by views in ferroelectrics

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Abstract
The Cryptococcus sp. S-2 carboxymethyl cellulase gene (CSCMCase) containing 1023 nucleotides and fused to acellulose-binding domain (CBD) which originated from cellobiohydrolase I of Trichoderma reesei (CSCMCase-CBD) wascloned and expressed in the methylotrophic yeast, Pichia pastoris. The fusion of CBD to CSCMCase resulted in a greatlyenhanced binding toward cellulose for CSCMCase-CBD compared with that for CSCMCase. This was useful for enzymepurification and enzyme immobilization. Furthermore, the recombinant CSCMCase-CBD showed high thermal stability afterincubating at 90oC for 3 h, whereas the recombinant CSCMCase rapidly lost its activity after incubating at 90oC for 1 h.
Reference Key
thongekkaew2009songklanakarinthermal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors
Journal ferroelectrics
Year 2009
DOI
DOI not found
URL
Keywords

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

No comments yet. Be the first to comment on this article.