thermal stability of cryptococcus sp. s-2 carboxymethyl cellulase (cscmcase) having a cellulose binding domain from a fungal exoglucanase: comparison to recombinant cscmcase

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ID: 213914
2009
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Abstract
The Cryptococcus sp. S-2 carboxymethyl cellulase gene (CSCMCase) containing 1023 nucleotides and fused to acellulose-binding domain (CBD) which originated from cellobiohydrolase I of Trichoderma reesei (CSCMCase-CBD) wascloned and expressed in the methylotrophic yeast, Pichia pastoris. The fusion of CBD to CSCMCase resulted in a greatlyenhanced binding toward cellulose for CSCMCase-CBD compared with that for CSCMCase. This was useful for enzymepurification and enzyme immobilization. Furthermore, the recombinant CSCMCase-CBD showed high thermal stability afterincubating at 90oC for 3 h, whereas the recombinant CSCMCase rapidly lost its activity after incubating at 90oC for 1 h.
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thongekkaew2009songklanakarinthermal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jantaporn Thongekkaew;Haruyuki Iefuji
Journal ferroelectrics
Year 2009
DOI
DOI not found
URL
http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-361-365.pdf
Keywords
thermal stability

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