integration of microcoils for on-chip immunosensors based on magnetic nanoparticles capture

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ID: 208313
2017
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Abstract
Immunoassays using magnetic nanoparticles (MNP) are generally performed under the control of permanent magnet close to the micro-tube of reaction. Using a magnet gives a powerful method for driving MNP but remains unreliable or insufficient for a fully integrated immunoassay on lab-on-chip. The aim of this study is to develop a novel lab-on-chip concept for high efficient immunoassays to detect ovalbumin (Biodefense model molecule) with microcoils employed for trapping MNP during the biofunctionalization steps. The objectives are essentially to optimize their efficiency for biological recognition by assuring a better bioactivity (antibodies-ovalbumin), and detect small concentrations of the targeted protein (~10 pg/mL). In this work, we studied the response of immunoassays complex function of ovalbumin concentration. The impact of MNP diameter in the biografting protocol was studied and permitted to choose a convenient MNP size for efficient biorecognition. We realized different immunoassays by controlling MNP in test tube and in microfluidic device using a permanent magnet. The comparison between these two experiments allows us to highlight an improvement of the limit of detection in microfluidic conditions by controlling MNP trapping with a magnet. Keywords: Bacteria, Lab-on-chip, ELISA, Magnetic nanoparticles, Ovalbumin, Microcoils, Fluorescent microscopy
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lefebvre2017sensingintegration Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Olivier Lefebvre;Claire Smadja;Emile Martincic;Marion Woytasik;Mehdi Ammar
Journal crystals
Year 2017
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2214180416301581
Keywords
engineering (general) civil engineering (general)

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