mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene

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ID: 207637
2010
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Abstract
The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for γ-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was used. The gene was under the control of the CaMV35S promoter region. GUS::GFP gene fusion was used as a reporter system and nptII served as a selectable marker gene. A high regeneration capacity of callus tissue under the selective pressure and successful GUS assay of transformed tissue were an indication of successful insertion of a transgene into mature embryo derived wheat tissue. .
Reference Key
vladislava2010archivesmature Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Galović Vladislava;Rausch T.;Gršić-Rausch Slobodanka
Journal gastrointestinal endoscopy
Year 2010
DOI
10.2298/ABS1003539G
URL
http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641003539G.pdf
Keywords
drought stress glutathione mature embryo

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