A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs.

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ID: 20673
2019
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Abstract
A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values against the initial concentration of pathogen in the sheep sample.
Reference Key
macori2019ajournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Macori, Guerrino;McCarthy, Siobhán C;Burgess, Catherine M;Fanning, Séamus;Duffy, Geraldine;
Journal Journal of microbiological methods
Year 2019
DOI
S0167-7012(19)30567-6
URL
Keywords Keywords not found

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