effect of xuezhikang capsule on the macrophage-derived foam cell formation and the expression of abac1 and abcg1
Clicks: 211
ID: 205239
2014
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
211 views
36 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #87 of 124 articles by views in frontiers in neurorobotics
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 124 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Objective To investigate the effects of Xuezhikang capsule on the THP-1 macrophage-derived foam cell formation and the cholesterol efflux mediated by ATP binding cassette transporter A1 (ABCA1) and ATP binding cassette transporter G1 (ABCG1). Methods THP-1 macrophages were induced to differentiate into macrophage-derived foam cells, and then they were divided into four groups: blank control group, ox-LDL group, Xuezhikang-treatment group, and vehicle group. The cells in four groups were co-incubated in culture medium, with addition of ox-LDL (100μg/ml), Xuezhikang (100mg/L ox- LDL+100μg/ml Xuezhikang) and vehicle solution (100mg/L ox-LDL+100μg/ml DMSO) respectively for 48 hours. The foam cells formation was observed with oil O staining. The contents of intracellular cholesterol and cholesterol ester in foam cells were determined. The mRNA and protein expression levels of ABCA1 and ABCG1 were determined by qRT-PCR and Western blotting, respectively. In addition, the cholesterol efflux was determined by liquid scintillation. Results After co-incubation with ox-LDL, abundant lipid droplets were found in THP-1 macrophages, which was consistent with the morphological feature of foam cells. Compared with 3 other groups, the foam cell formation was attenuated by the administration of Xuezhikang. Compared with blank control group and vehicle group, the cellular lipid contents in Xuezhikang group significantly decreased (P<0.01), while the ABCA1 and ABCG1 expression significantly elevated (P<0.05), and the cholesterol efflux significantly promoted by Xuezhikang treatment (P<0.05). Conclusions Xuezhikang may inhibit the foam cell formation in vitro by means of reducing intracellular cholesterol level. The cholesterol efflux is enhanced by Xuezhikang, possibly due to up-regulated expression of ABCA1 and ABCG1 in both mRNA and protein level.
DOI: 10.11855/j.issn.0577-7402.2014.02.07
| Reference Key |
wang2014medicaleffect
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Jun WANG;Wei-min JIANG;Yong ZHONG;Ping GU;Li-jun WANG;Jian-bin GONG;Shi-sen JIANG |
| Journal | frontiers in neurorobotics |
| Year | 2014 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.