epidemiologia molecular de estirpes de mycobacterium tuberculosis isoladas em lisboa: descrição preliminar de um genótipo único em portugal
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ID: 203325
1996
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Abstract
Resumo: Com vista a uma avaliação da importâncio da epidemiologi da tuberculose na População portuguesa, analisaram-se os perfis de restrição de DNA isolado de estirpes de Mycobacterium tuberculosis. estirpes esas oriundas de dois Hospital de Lisboa, Portugal pais ondea tuberculose é endemic. O DNA de 155 estirpes de Mycobacterium tuberculosis isoladas de 147 doentes durante os anos de 1992 e 1993 (incluindo 27 estirpes isoladas em Cabinda, Angola, durante o mesmo period), foi submetido à análise por Restriction Fragment Length Polymorphism (RFLP), usando a sequência de inserção IS6110 como sonda. Identificámos 108 perfis distintos, e 30 estirpes foram agrurpadas em grupos de pelo menos 2 estirpes iguais. Um destes grupos, cujas estripes apresentaram 9 bandas em locais particulares, parece ser um genótipo único português. ABSTRACT: We conducted a DNA llngerprinttog analysts of Mycobacterium tuberculosis strains in Lisbon, Portugal a country in wich tuberculosis is endemic in order to evaluate the importance of microepidemics in the maintenance of the disease within the population. The genetic polymorphim of 155 strains or Mycobacterium tuberculosis isolated from 147 patients during 1992 and 1993 (including 27 strains collected from Cabinda, Angola, during the some period) were subjected to DNA restriction fragment length polymorphism (RFLP) analysis, using the insertion sequence IS6110 as a probe. 108 different IS6110 RFLP types were identifed, and 30 strains were grouped in clusters, by computer-assisted analysts of DNA fingerprints. One of this clusters, presenting 9 bands in a particular place, seems to be a portuguese single genotype. Palavras chave: Mycobacterium tuberculosis, RFLP, Key words: Mycobacterium tuberculosis, RFLP
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portugal1996revistaepidemiologia
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| Authors | ;Isabel Portugal |
| Journal | the journal of applied psychology |
| Year | 1996 |
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