plant regeneration from protoplast of brazilian citrus cultivars

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ID: 196931
2000
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Ranked #28 of 193 articles by views in proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006

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Abstract
A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract. Well defined embryos were germinated in modified MT medium with addition of GA3 (2.0 muM) and malt extract (500 mg/L). Plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified MT medium with addition of malt extract (500 mg/L), BAP (1.32 muM), NAA (1.07 muM) and coconut water (10 mL/L). Plantlets were transferred to root medium. Rooted plants were transferred to a greenhouse for further adaptation and development.
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Authors ;GLORIA FERNANDA JANUZZI MENDES DA;MOURÃO FILHO FRANCISCO DE ASSIS ALVES;MENDES BEATRIZ MADALENA JANUZZI
Journal proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006
Year 2000
DOI
DOI not found
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400008
Keywords
polyethylene glycol rootstock tissue cultureagriculture (general)

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