seizure-like activity in hyaluronidase-treated dissociated hippocampal cultures

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ID: 196224
2013
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Ranked #57 of 394 articles by views in macromolecular bioscience

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Abstrak
The extracellular matrix (ECM) plays an important role in use-dependent synaptic plasticity. Hyaluronic acid (HA) is the backbone of the neural ECM, which has been shown to modulate -amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA) receptor mobility, paired-pulse depression, L-type voltage-dependent Ca2+ channel (L-VDCC) activity, long-term potentiation and contextual fear conditioning. To investigate the role of HA in the development of spontaneous neuronal network activity, we used microelectrode array recording and Ca2+ imaging in hippocampal cultures enzymatically treated with hyaluronidase. Our findings revealed an appearance of epileptiform activity 9 days after hyaluronidase treatment. The treatment transformed the normal network firing bursts and Ca2+ oscillations into long-lasting superbursts and superoscillations with durations of 11-100 seconds. The changes in Ca2+ transients in hyaluronidase-treated neurons were more prominent then in astrocytes and preceded changes in electrical activity. The Ca2+ superoscillations could be suppressed by applying the L-VDCC blocker diltiazem, whereas the neuronal firing superbursts could be additionally suppressed by an 6-cyano-7-nitroquinoxaline-2,3-dione as an antagonist of AMPA/kainate receptors. These results suggest that changes in the expression of HA can be epileptogenic and that hyaluronidase treatment in vitro provides a robust model for the dissection of the underlying mechanisms.
Kunci Referensi
evedunova2013frontiersseizure-like Gunakan kunci ini untuk mengutip otomatis dalam naskah saat menggunakan SciMatic Manuscript Manager atau Thesis Manager
Penulis ;Maria eVedunova;Maria eVedunova;Tatiana eSakharnova;Tatiana eSakharnova;Elena eMitroshina;Elena eMitroshina;Maya ePerminova;Maya ePerminova;Yuri eZakharov;Alexey ePimashkin;Alexander eDityatev;Alexander eDityatev;Alexander eDityatev;Irina eMukhina;Irina eMukhina
Jurnal macromolecular bioscience
Tahun 2013
DOI
10.3389/fncel.2013.00149
URL
http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00149/full
Kata Kunci
hyaluronic acid seizure extracellular matrix microelectrode array neuropsychiatry biological psychiatry neuronal networkneurosciences

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