viabilidade espermática do sêmen de piracanjuba (brycon orbignyanus) resfriado a 4°c spermatic viability of piracanjuba (brycon orbignyanus) semen cooled at 4°c

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ID: 194835
2004
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Abstract
Os efeitos de três meios diluidores (BTS+KCl - D1, BTS+2KCL - D2 e BTS+Citrato de Sódio - D3), considerando três diferentes tempos de resfriamento (0, 72 e 144 horas), foram analizados no sêmen de Piracanjuba (Brycon orbignyanus) a 4°C. Testou-se, ainda, a utilidade do meio diluidor BTS (Beltsville Thawing Solution), adicionado a citrato de sódio e cloreto de potássio, na manutenção da imobilidade dos espermatozóides e na conservação de características do sêmen. Resultados satisfatórios foram encontrados até 144 horas de resfriamento. A motilidade espermática média do sêmen in natura foi de 94 ± 5,48%, com duração média de 55,6 ± 32,25 segundos (s). Para o sêmen diluído, as médias da motilidade e sua duração foram de 76,00 ± 1,86% e 56,6 ± 6,54 s, 77,00 ± 1,86% e 54,6 ± 6,54 s, e 74,33 ± 1,86% e 75,0 ± 6,54 s, respectivamente, para os diluidores D1, D2 e D3. Não houve diferenças significativas entre os diluidores, que apresentaram efeitos semelhantes nos diferentes tempos. As análises nos tempos 0 e 72 horas mostraram motilidades espermáticas superiores àquelas nas 144 horas, embora as durações da motilidade espermática não tenham diferido nesses tempos. A concentração espermática média do sêmen foi de 8,21 ± 2,26 X 10(9) sptz/mL. Algumas amostras evidenciaram motilidade espermática antes da ativação do sêmen (pré-ativação), principalmente a partir das 96 horas de resfriamento. O meio diluidor BTS pode ser recomendado para conservação de sêmen de piracanjuba (B. orbignyanus), desde que sua osmolaridade seja elevada.
The experiment was conducted in order to evaluate the effect of 3 diluents BTS+KCl - D1, BTS+2KCL - D2 and BTS+Sodium Citrate - D3) and three different times (hour 0, 72 hours and 144 hours) in Piracanjuba (Brycon orbignyanus) semen at 4°C. The assay aimed to test usefulness of BTS (Betsville Thawing Solution) diluent medium, added to Sodium citrate and Potassium clorate, in maintenance of spermatic immobility and of its conditions to conserve semen characteristics as spermatic motility rate and duration. Satisfactory results were found until at 144 hours of cooling for this specie. The mean spermatic motility in the in natura state was 94 ± 5.48% with mean duration of 55.6 ± 32.25 seconds (s). For diluted semen, motility and duration means were 76.00 ± 1.86% and 56.6 ± 6.54 s, 77.00 ± 1.86% and 54.6 ± 6.54 s, and 74.33 ± 1.86% and 75.0 ± 6.54 s, respectivaly, for diluents D1, D2 and D3, in this order. Significant differences were not observed among diluents and they showed similar effects in at different times. At 0 and 72 h of analysis showed higher spermatic motilities than that ones at 144 h, but durations of spermatic motility did not show differences in these times. The semen mean spermatic concentration was 8.21 ± 2.26 X 10(9) sptz/mL. Some samples showed spermatic motility before semen activation (fore-activation) principally after 96 hours of cooling. Thus, BTS medium diluent may be recommended for fish semen conservation, since its osmolarity is elevated.
Reference Key
murgas2004revistaviabilidade Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Luis David Solis Murgas;Aléssio Batista Miliorini;Renan Toledo Franciscatto;Alexandre Nízio Maria
Journal european journal of lipid science and technology
Year 2004
DOI
10.1590/S1516-35982004000600001
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600001
Keywords
semen cooling

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