physical mapping of 5s and 18s-5.8s-26s rna gene families in polyploid series of cenchrus ciliaris linnaeus, 1771 (poaceae)

Clicks: 167
ID: 193424
2012
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Abstract
The Buffelgrass (Cenchrus ciliaris L., Poaceae) is one of the most important pasturage grasses due to its high productivity and good forage qualities. This species possess a high adaptability to bioclimatic constraints of arid zones and may be used for the restoration of degraded arid ecosystems. Tunisian populations present three ploidy levels (4x, 5x and 6x) with a basic chromosome number x=9. This study reported for the first time the distribution of the ribosomal genes (rRNA) for pentaploid and hexaploid cytotypes of C. ciliaris. Molecular cytogenetic study using double fluorescence in situ hybridization has shown that the two rDNA families, 5S and 18S-5.8S-26S (18S), displayed intraspecific variation in number of loci among different ploidy levels. Each ploidy level was characterized by specific number of both 5S and 18S rDNA loci (two loci in tetraploid, five in pentaploid and six in hexaploid level). For three studied cytotypes (4x, 5x and 6x) all 5S rDNA loci were localized on the subcentromeric region of chromosomes, while 18S loci were situated on the telomeric region of short chromosome arms. Data of the FISH experiments show proportional increase of ribosomal loci number during polyploidization processes.
Reference Key
kharrat-souissi2012comparativephysical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Amina Kharrat-Souissi;Sonja Siljak-Yakovlev;Fatima Pustahija;Mohamed Chaieb
Journal Vascular health and risk management
Year 2012
DOI
10.3897/compcytogen.v6i3.3380
URL
http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1762
Keywords
genetics

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