genome-wide analysis of dna methylation, copy number variation, and gene expression in monozygotic twins discordant for primary biliary cirrhosis
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ID: 192866
2014
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Abstract
Primary biliary cirrhosis (PBC) is an uncommon autoimmune disease with a homogeneous clinical phenotype that reflects incomplete disease concordance in monozygotic (MZ) twins. We have taken advantage of a unique collection consisting of genomic DNA and mRNA from peripheral blood cells of female MZ twins (n=3 sets) and sisters of similar age (n=8 pairs) discordant for disease. We performed a genome-wide study to investigate differences in (i) DNA methylation (using a custom tiled 4-plex array containing tiled 50-mers 19,084 randomly chosen methylation sites), (ii) copy number variation (CNV) (with a chip including markers derived from the 1000 Genomes Project, all three HapMap phases, and recently published studies), and/or (iii) gene expression (by whole-genome expression arrays). Based on the results obtained from these three approaches we utilized quantitative PCR to compare the expression of candidate genes. Importantly, our data support consistent differences in discordant twins and siblings for the (i) methylation profiles of 60 gene regions, (ii) CNV of 10 genes, and (iii) the expression of 2 interferon-dependent genes. Quantitative PCR analysis showed that 17 of these genes are differentially expressed in discordant sibling pairs. In conclusion, we report that MZ twins and sisters discordant for PBC manifest particular epigenetic differences and highlight the value of the epigenetic study of twins.
| Reference Key |
eselmi2014frontiersgenome-wide
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| Authors | ;Carlo eSelmi;Carlo eSelmi;Francesca eCavaciocchi;Francesca eCavaciocchi;Ana eLleo;Cristina eCheroni;Raffaele eDe Francesco;Simone A. Lombardi;Maria eDe Santis;Maria eDe Santis;Francesca eMeda;Maria Gabriella eRaimondo;Chiara eCrotti;Marco eFolci;Luca eZammataro;Marlyn J. Mayo;Nancy eBach;Shinji eShimoda;Stuart eGordon;Monica eMiozzo;Pietro eInvernizzi;Pietro eInvernizzi;Mauro ePodda;Rossana eScavelli;Michelle R. Martin;Michael Forrest Seldin;Michael Forrest Seldin;Janine M. LaSalle;M. Eric eGershwin |
| Journal | sudebno-meditsinskaia ekspertiza |
| Year | 2014 |
| DOI |
10.3389/fimmu.2014.00128
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| URL | |
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