effect of different broad waveband lights on membrane lipids of a cyanobacterium, synechococcus sp., as determined by uplc-qtof-ms and vibrational spectroscopy

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ID: 188803
2016
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Abstract
Differential profile of membrane lipids and pigments of a Synechococcus sp. cyanobacterial strain cells exposed to blue, green, red and white light are determined by means of liquid chromatography and mass spectrometry or diode array detection. Raman and ATR-IR spectra of intact cells under the diverse light wavebands are also reported. Blue light cells exhibited an increased content of photosynthetic pigments as well as specific species of membrane glycerolipids as compared to cells exposed to other wavebands. The A630/A680 ratio indicated an increased content of phycobilisomes (PBS) in the blue light-exposed cells. Some differences in the protein conformation between the four light waveband-exposed cells were deduced from the variable absorbance at specific wavenumbers in the FT-Raman and ATR-FTIR spectra, in particular bands assigned to amide I and amide II. Bands from 1180 to 950 cm−1 in the ATR-FTIR spectrum suggest degraded outer membrane polysaccharide in the blue light-exposed cells.
Reference Key
montero2016biologyeffect Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Olimpio Montero;Marta Velasco;Aurelio Sanz-Arranz;Fernando Rull
Journal Journal of microencapsulation
Year 2016
DOI
10.3390/biology5020022
URL
http://www.mdpi.com/2079-7737/5/2/22
Keywords
lipids raman vibrational spectroscopy pigments uplc-ms ir

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