raav vectors as safe and efficient tools for the stable delivery of genes to primary human chondrosarcoma cells in vitro and in situ

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ID: 188361
2012
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Abstract
Treatment of chondrosarcoma remains a major challenge in orthopaedic oncology. Gene transfer strategies based on recombinant adenoassociated viral (rAAV) vectors may provide powerful tools to develop new, efficient therapeutic options against these tumors. In the present study, we tested the hypothesis that rAAV is adapted for a stable and safe delivery of foreign sequences in human chondrosarcoma tissue by transducing primary human chondrosarcoma cells in vitro and in situ with different reporter genes (E. coli lacZ, firefly luc, Discosoma sp. RFP). The effects of rAAV administration upon cell survival and metabolic activities were also evaluated to monitor possibly detrimental effects of the gene transfer method. Remarkably, we provide evidence that efficient and prolonged expression of transgene sequences via rAAV can be safely achieved in all the systems investigated, demonstrating the potential of the approach of direct application of therapeutic gene vectors as a means to treat chondrosarcoma.
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madry2012sarcomaraav Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Henning Madry;Jagadeesh K. Venkatesan;Gertrud Schmitt;Sarah Schetting;Myriam Ekici;Dieter Kohn;Magali Cucchiarini
Journal the journal of physiology
Year 2012
DOI
10.1155/2012/347417
URL
http://dx.doi.org/10.1155/2012/347417
Keywords
neoplasms Oncology including cancer and carcinogens tumors

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