molecular markers. amplified fragment length polymorphism

Clicks: 370
ID: 181347
2005
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Abstract
Amplified Fragment Length Polymorphism molecular markers (AFLPs) has been developed combining procedures of RFLPs and RAPDs molekular markers, i.e. the first step is restriction digestion of the genomic DNA that is followed by selective amplification of the restricted fragments. The advantage of the AFLP technique is that it allows rapid generation of a large number of reproducible markers. The reproducibility of AFLPs markers is assured by the use of restriction site-specific adapters and adapter-specific primers for PCR reaction. Only fragments containing the restriction site sequence plus the additional nucleotides will be amplified and the more selected nucleotides added on the primer sequence the fewer the number of fragments amplified by PCR. The amplified products are normally separated on a sequencing gel and visualized after exposure to X-ray film or by using fluorescent labeled primers. AFLP shave proven to be extremely proficient in revealing diversity at below the species level. A disadvantage of AFLP technique is that AFLPs are essentially a dominant marker system and not able to identify heterozygotes.
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novo2005selekcijamolecular Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Pržulj Novo;Perović Dragan
Journal british journal for the history of science
Year 2005
DOI
DOI not found
URL
http://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2005/0354-58810504069P.pdf
Keywords
rapd scar aflp molecular markers marker-assisted selection DaF disadvantages of rapdagriculture (general)biotechnology

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