the nuclear cap-binding complex mediates meiotic silencing by unpaired dna

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ID: 174637
2017
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Abstract
In the filamentous fungus Neurospora crassa, cross walls between individual cells are normally incomplete, making the entire fungal network vulnerable to attack by viruses and selfish DNAs. Accordingly, several genome surveillance mechanisms are maintained to help the fungus combat these repetitive elements. One of these defense mechanisms is called meiotic silencing by unpaired DNA (MSUD), which identifies and silences unpaired genes during meiosis. Utilizing common RNA interference (RNAi) proteins, such as Dicer and Argonaute, MSUD targets mRNAs homologous to the unpaired sequence to achieve silencing. In this study, we have identified an additional silencing component, namely the cap-binding complex (CBC). Made up of cap-binding proteins CBP20 and CBP80, CBC associates with the 5′ cap of mRNA transcripts in eukaryotes. The loss of CBC leads to a deficiency in MSUD activity, suggesting its role in mediating silencing. As confirmed in this study, CBC is predominantly nuclear, although it is known to travel in and out of the nucleus to facilitate RNA transport. As seen in animals but not in plants, CBP20’s robust nuclear import depends on CBP80 in Neurospora. CBC interacts with a component (Argonaute) of the perinuclear meiotic silencing complex (MSC), directly linking the two cellular factors.
Reference Key
decker2017g3:the Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Logan M. Decker;Hua Xiao;Erin C. Boone;Michael M. Vierling;Benjamin S. Shanker;Shanika L. Kingston;Shannon F. Boone;Jackson B. Haynes;Patrick K.T. Shiu
Journal separation and purification technology
Year 2017
DOI
10.1534/g3.116.038679
URL
http://g3journal.org/lookup/doi/10.1534/g3.116.038679
Keywords
meiosis neurospora crassa

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