total lipid and fatty acid accumulation during basidiospore formation in the ectomycorrhizal fungus pisolithus sp. acúmulo de lipídeos totais e de ácidos graxos durante a formação de basidiósporos no fungo ectomicorrízico pisolithus sp.

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ID: 172436
2008
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Abstract
The basidiospores of Pisolithus sp. contain large amounts of lipids, indicating provision for future germination in the host rhizosphere. However, the accumulation, composition, and mobilization of lipids during formation and germination of these spores are largely unknown. In this study, lipid storage and fatty acid composition during basidiosporogenesis were analyzed in fresh basidiocarps using bright-field microscopy and gas chromatography. Abundant lipid bodies are found in the hyphae, basidia, and basidiospores of fungal basidiocarps. This evidences a considerable C transport in the basidiocarp to meet the C demand during basidiospore formation. Fatty acid composition analysis revealed the presence of 24 compounds with chains of 9 to 18 C atoms, either saturated or insaturated, with one or two insaturations. The fatty acid composition and content varied according to the developmental stage of the peridioles. In free basidiospores, the predominant compounds were 16:0, 16:1w5c, 18:1w9c, and 18:2w6,9c/18:0ante, at concentrations of 76, 46, 192, and 51 µg g-1 dry matter, respectively. Our results indicate that oleic acid is the major constituent of lipid reserves in Pisolithus sp. basidiospores. Further studies are being conducted to determine the factors that induce lipid mobilization during spore germination.
Os basidiósporos de Pisolithus sp. contêm grande quantidade de lipídeos, indicando provisão para futura germinação na rizosfera da planta hospedeira. No entanto, pouco se conhece a respeito do acúmulo, da composição e da mobilização dos lipídeos durante a formação e germinação dos esporos. Neste trabalho, o armazenamento de lipídeos e a análise da composição em ácidos graxos durante a basidiosporogênese foram estudados utilizando-se técnicas de microscopia de luz e cromatografia gasosa. O basidiocarpo fúngico apresentou abundantes corpos lipídicos no interior de suas hifas, basídios e basidiósporos. Esse fato evidencia considerável transporte de C dentro do basidiocarpo para suprir a demanda de C durante a formação dos esporos. A composição de ácidos graxos revelou a presença de 24 compostos com cadeias variando de 9 a 18 átomos de C, sendo tanto saturados ou contendo uma ou duas insaturações. A composição de ácidos graxos variou de acordo com o estádio de desenvolvimento dos peridíolos. Nos basidiósporos livres, os compostos predominantes foram 16:0, 16:1w5c, 18:1w9c e 18:2w6,9c/18:0ante, com concentrações de 76, 46, 192 e 51 µg g-1 do ácido graxo de matéria seca, respectivamente. Esses resultados indicam que o ácido oléico é o principal constituinte das reservas lipídicas nos basidiósporos de Pisolithus sp. Outros trabalhos estão sendo realizados para determinar os fatores que desencadeiam a mobilização de lipídeos durante a germinação de esporos.
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Authors ;André Narvaes da Rocha Campos;Maurício Dutra Costa;Marcos Rogério Tótola;Arnaldo Chaer Borges
Journal Revista de saude publica
Year 2008
DOI
10.1590/S0100-06832008000400017
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000400017
Keywords
ectomycorrhiza basidiomycota carbon storage fatty acidsagriculture (general) basidiosporogenesis

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