highly efficient production of aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate
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ID: 171330
2015
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Abstract
Triticale (Triticosecale, Wittmak) an important industrial crop, with high
grain yield, containing high amounts of starch, proteins and also major and
minor mineral elements, is not sufficiently utilized yet. The simultaneous
production of α-amylase and glucoamylase isoforms by Aspergillus niger on
triticale grains, without any nutritive supplements, has been developed,
optimized and scaled up 10 fold for the first time. The specific combination
of examined effects led to the production of a novel glucoamylase isoform.
Reduction of particle size, increase of oxygen availability and substrate
height lead to increase of amylases production of 30%. Reduction of relative
humidity from 65% to 30% increased glucoamylase production 2 times and
α-amylase production for 30%. Peak production of α-amylase (158 U/g) and
glucoamylase (170 U/g) were obtained in erlenmayers and in scaled-up trays.
The obtained A. niger amylase cocktail was more efficient in raw starch
hydrolysis from wheat flour, 29% more efficient in glucose formation and 10%
more efficient in total reducing sugar formation, than commercially
available amylase cocktail SAN Super 240L widely used in industry. [Projekat
Ministarstva nauke Republike Srbije, br. 172048]
| Reference Key |
biljana2015journalhighly
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|---|---|
| Authors | ;Dojnov Biljana;Grujić Marica;Vujčić Zoran |
| Journal | meditsinskaia radiologiia |
| Year | 2015 |
| DOI |
10.2298/JSC150317041D
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| URL | |
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