mix-and-diffuse serial synchrotron crystallography

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ID: 169987
2017
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Abstract
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.
Reference Key
beyerlein2017iucrjmix-and-diffuse Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Kenneth R. Beyerlein;Dennis Dierksmeyer;Valerio Mariani;Manuela Kuhn;Iosifina Sarrou;Angelica Ottaviano;Salah Awel;Juraj Knoska;Silje Fuglerud;Olof Jönsson;Stephan Stern;Max O. Wiedorn;Oleksandr Yefanov;Luigi Adriano;Richard Bean;Anja Burkhardt;Pontus Fischer;Michael Heymann;Daniel A. Horke;Katharina E. J. Jungnickel;Elena Kovaleva;Olga Lorbeer;Markus Metz;Jan Meyer;Andrew Morgan;Kanupriya Pande;Saravanan Panneerselvam;Carolin Seuring;Aleksandra Tolstikova;Julia Lieske;Steve Aplin;Manfred Roessle;Thomas A. White;Henry N. Chapman;Alke Meents;Dominik Oberthuer
Journal european journal of orthopaedic surgery & traumatology : orthopedie traumatologie
Year 2017
DOI
10.1107/S2052252517013124
URL
http://scripts.iucr.org/cgi-bin/paper?S2052252517013124
Keywords
X-ray crystallography protein structure drug discovery lysozymescience

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