vitrificação de ovócitos desnudados ou não e previamente maturados in vitro cryopreservation of bovines oocytes desnuded or not and previously in vitro matured
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2004
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Abstract
Objetivou-se avaliar os efeitos da vitrificação de ovócitos maturados in vitro de bovinos, utilizando o etilenoglicol (EG) associado a trehalose e polivinilpirrolidona (PVP). Utilizaram-se ovócitos provenientes de ovários de vacas abatidas em matadouro, distribuídos aleatoriamente em três tratamentos. Tratamento 0 (T0 - testemunha): ovócitos não desnudados e não congelados. Tratamento 1 (T1): vitrificação de ovócitos com cumulus oophorus e maturados in vitro. Tratamento 2 (T2): vitrificação de ovócitos desnudados e maturados in vitro. A porcentagem de ovócitos recuperados e com morfologia normal após a desvitrificação foi diferente entre T1 e T2 (94,7 e 76,8%; 69,5 e 49,85%, para T1 e T2, respectivamente). Após a reidratação, os ovócitos vitrificados foram fecundados e cultivados in vitro por sete dias. Foi verificada, em nível ultra-estrutural, liberação prematura dos grânulos corticais em ovócitos vitrificados. As taxas de fecundação e de clivagem foram diferentes entre os tratamentos (56,2; 41,7 e 12,5%; 36,3; 0,0 e 0,0% para T0, T1 e T2, respectivamente). Apenas no T0 foram obtidos mórulas e blastocistos (34,5%). Estes resultados indicam que o procedimento de vitrificação, segundo os protocolos utilizados, não é indicado para a criopreservação de ovócitos maturados de bovinos.
This study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (EG) associated to trehalose and polyvinylpyrrolidone (PVP), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. Treatment 0 (T0 - control): oocytes that were desnuded and not vitrified. Treatment 1 (T1): cryopreservation of in vitro matured oocytes with cumulus oophorus. Tratamento 2 (T2): cryopreservation of in vitro matured desnuded oocytes. The percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for T1 and T2, respectively). The main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. Later, all normal oocytes were fecundated and cultivated at 38.5ºC in atmosphere with 5% CO2 for seven days. The fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for T0, T1 and T2, respectively). Morulas and blastocysts were obtained only in T0 (34.5%). These results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
This study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (EG) associated to trehalose and polyvinylpyrrolidone (PVP), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. Treatment 0 (T0 - control): oocytes that were desnuded and not vitrified. Treatment 1 (T1): cryopreservation of in vitro matured oocytes with cumulus oophorus. Tratamento 2 (T2): cryopreservation of in vitro matured desnuded oocytes. The percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for T1 and T2, respectively). The main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. Later, all normal oocytes were fecundated and cultivated at 38.5ºC in atmosphere with 5% CO2 for seven days. The fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for T0, T1 and T2, respectively). Morulas and blastocysts were obtained only in T0 (34.5%). These results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
| Reference Key |
fagundes2004revistavitrificao
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|---|---|
| Authors | ;Letícia Martins Fagundes;Eduardo Paulino da Costa;Ciro Alexandre Alves Torres;Wald'ma Sobrinho Amaral Filha;Trícia Osório da Silva;Marilú Martins Gioso |
| Journal | european journal of lipid science and technology |
| Year | 2004 |
| DOI |
10.1590/S1516-35982004000500004
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| URL | |
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