in vitro evaluation of the antibacterial and anti-inflammation activities of clausena lansium (lour.) skeels

Crude extracts of twigs and roots of Clausena lansium (Lour.) Skeels were separated and purified using repeated silica gel column chromatography to yield 12 compounds identified as xanthotoxol (1), imperatorin (2), heraclenol (3), heraclenin (4), wampetin (5), indicolactonediol (6), murrayanine (7), O-demethylmurrayanine (8), indizoline (9), 3-formyl-6-methoxycarbazole (10), lansine (11) and glycozolidal (12). All pure compounds were tested for their antibacterial and anti-inflammation activities using disc diffusion method and enzyme-linked immunosorbent assay (ELISA), respectively. At a concentration of 50 µg/mL, three carbazole alkaloid components (compounds 10, 11, and 12) demonstrated moderate antibacterial activity against the periodontopathic bacteria, Porphyromonas gingivalis. Compound 10 and the crude extract of twig revealed impressive antiinflammation potency. From these results, selected carbazole alkaloid compounds from Clausena lansium might be potential raw products in generating new anti-inflammation and antibacterial agent used as an adjunctive medication in treating periodontal disease.