the first occurrence of stem canker on oilseed rape caused by leptosphaeria biglobosa in serbia

Clicks: 242
ID: 162309
2016
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Popular

Ranked #28 of 41 articles by views in Preventive medicine

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
In October 2010 the occurrence of the stem canker symptoms in rapeseed was observed at the locality Rimski Šančevi in Serbia. Several strains of fungi were isolated from the stem. Morphological characteristics of the isolates were studied on a PDA medium at 25±1oC: growth rate, colour, shape and appearance of colonies and the edge of the colony, the pigment, size, colour and shape of pycnidiospores and pycnidia in 10 isolates (isolated in Serbia K-111, IK-112, K-113, K-114, K-115, K-116, IK-117, K-118, K-119, and K-120). All tested strains had fast growth, the regular form of colonies and poor sporulation on nutrient medium. Pycnidiospores are unicellular, hyaline, and mostly straight, with or without a drop of oil. Molecular identification was performed by the application the PCR technique using primers PN3/PN10. In addition to these 10 isolates, two reference strains obtained from the Centre for Agricultural Studies, Rothamsted, UK, which are marked with L. m (Leptosphaena maculanS), L. b (Leptosphaeria biglobosa) and 7 reference isolates originating from Serbia, which are marked K-7, St-16, GS-25, L-5, C-3, LJ-2, S-1 were used. On the basis of DNA amplification with primers PN3 and PN10 tape length was about 580 bp for isolates (L.b, IK-111, K-112, K-113, K-114, K-115, K-116, K-117, K-118, K-119, K-120) while that was 560 bp for other group of isolates (L.m, K-7, St-16, GS-25, L-5, C-3, LJ- 2, S-1).This study showed that 10 isolates (K-111, K-112, K-113, IK- 114, K-115, K-116, K-117, K-118, K-119, K-120) isolated from the stem of rapeseed belong to the species Leptosphaeria biglobosa.
Reference Key
petar2016ratarstvothe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Mitrović Petar;Marjanović-Jeromela Ana;Trkulja Vojislav;Milovac Željko;Terzić Sreten
Journal Preventive medicine
Year 2016
DOI
10.5937/ratpov53-8997
URL
http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2016/1821-39441602053M.pdf
Keywords
oilseed rape morphological characteristics

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.