a genetic screen for mutants with supersized lipid droplets in caenorhabditis elegans

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ID: 154062
2016
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Abstract
To identify genes that regulate the dynamics of lipid droplet (LD) size, we have used the genetically tractable model organism Caenorhabditis elegans, whose wild-type LD population displays a steady state of size with an upper limit of 3 μm in diameter. From a saturated forward genetic screen of 6.7 × 105 mutagenized haploid genomes, we isolated 118 mutants with supersized intestinal LDs often reaching 10 μm. These mutants define nine novel complementation groups, in addition to four known genes (maoc-1, dhs-28, daf-22, and prx-10). The nine groups are named drop (lipid droplet abnormal) and categorized into four classes. Class I mutants drop-5 and drop-9, similar to prx-10, are up-regulated in ACS-22-DGAT-2-dependent LD growth, resistant to LD hydrolysis, and defective in peroxisome import. Class II mutants drop-2, drop-3, drop-6, and drop-7 are up-regulated in LD growth, are resistant to LD hydrolysis, but are not defective in peroxisome import. Class III mutants drop-1 and drop-8 are neither up-regulated in LD growth nor resistant to LD hydrolysis, but seemingly up-regulated in LD fusion. Class IV mutant drop-4 is cloned as sams-1 and, different to the other three classes, is ACS-22-independent and hydrolysis-resistant. These four classes of supersized LD mutants should be valuable for mechanistic studies of LD cellular processes including growth, hydrolysis, and fusion.
Reference Key
li2016g3:a Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Shiwei Li;Shibin Xu;Yanli Ma;Shuang Wu;Yu Feng;Qingpo Cui;Lifeng Chen;Shuang Zhou;Yuanyuan Kong;Xiaoyu Zhang;Jialei Yu;Mengdi Wu;Shaobing O. Zhang
Journal separation and purification technology
Year 2016
DOI
10.1534/g3.116.030866
URL
http://g3journal.org/lookup/doi/10.1534/g3.116.030866
Keywords
peroxisome lipid droplet

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