Generation of a human control PBMC derived iPS cell line TUSMi001-A from a healthy male donor of Han Chinese genetic background

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2017
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Abstract
A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. In the studies of disease mechanism, the iPSC line can be used as a control.
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wang2017generationstem Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Wang, Ying;Zhang, Yue;Zhang, Jing;Lu, Jing;Yang, Chaowen;Zhao, Jian;Li, Gang;Liu, Zhongmin;Lei, Ying;
Journal Stem cell research
Year 2017
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S1873506117302118
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