insights from the cold transcriptome and metabolome of dendrobium officinale: global reprogramming of metabolic and gene regulation networks during cold acclimation

Klik: 369
ID: 144383
2016
PDF Gratis
Metrik Kualitas & Kinerja Artikel
Kualitas Keseluruhan
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
Penilaian Kualitas AI
Belum dianalisis
Readership in this journal
Steady

Ranked #108 of 812 articles by views in phytochemistry letters

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 812 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstrak
Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 oC to -2 oC resulted in significant increase（P<0.01）in antioxidant activities and electrolyte leakage during 24 h. The fitness CA piont of 0 oC and control (20 oC) during 20 h were firstly obtained according to physiological analyses. Subsequently, massive transcriptome and metabolome reprogramming occurred during CA. The gene to metabolite network demonstrated that the CA associated processes are highly energy demanding through activating hydrolysis of sugars, amino acids catabolism and citrate cycle. The expression levels of 2,767 genes were significantly affected by CA, including 153-fold upregulation of CBF transcription factor, 56-fold upregulation of MAPKKK16 protein kinase. Moreover, the gene interaction and regulation network analysis revealed that the CA as an active process, was regulated at the transcriptional, post-transcriptional, translational and post-translational levels. Our findings highligted a comprehensive regulatory mechanism including cold signal transduction, transcriptional regulation and gene expression, which contributes a deeper understanding of the highly complex regulatory program during CA in DOKM. Some marker genes identified in DOKM seedlings will allow us to understand the role of each individual during CA by further functional analyses.
Kunci Referensi
wu2016frontiersinsights Gunakan kunci ini untuk mengutip otomatis dalam naskah saat menggunakan SciMatic Manuscript Manager atau Thesis Manager
Penulis ;Zhi-Gang Wu;Wu Jiang;Song-lin Chen;Nitin Mantri;Zheng-Ming Tao;Cheng-Xi Jiang
Jurnal phytochemistry letters
Tahun 2016
DOI
10.3389/fpls.2016.01653
URL
http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01653/full
Kata Kunci
transcriptome cold acclimation signal transduction metabolic network dendrobium officinaleplant culture

Sitasi

Tidak ada sitasi yang ditemukan. Untuk menambahkan sitasi, hubungi admin di info@scimatic.org

Komentar

Belum ada komentar. Jadilah yang pertama berkomentar pada artikel ini.