oral morphine consumption reduces lens development in rat embryos

El consumo oral de morfina reduce el desarrollo del cristalino en embriones de rata.

Clicks: 402
ID: 143530
2012
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Abstract
Objective: Consumption of morphine, during pregnancy, in addition to inducing defects in the mother’s nervous system function, caused defects or delays in the formation and evolution of embryonic visual system. In the present study, changes in lens development was assessed in embryos exposed in utero to morphine. Material and Methods: Female Wistar rats (250-300 g) were mated with male rats and pregnancy was determined by sperm observation in vaginal smear. This day was considered as embryonic day zero (E0). The females were then divided randomly into the experimental and the control groups. The control group received tap water and the experimental group received morphine (0.05 mg/ml) in their water. On embryonic day 13 ( E13), blood samples were collected from the retro-orbital sinus of all animals for plasma corticosterone detection. On embryonic day 17(E17), the animals were killed by an overdose of chloroform and the embryos were taken out surgically. The embryos were fixed in 10% formalin for 30 days. At this time, the head of the embryos were removed for tissue processing and Hematoxylin- Eosin (H&E) staining. The samples were evaluated using light microscope and MOTIC software. Results: Our data indicated that plasma corticosterone level was dramatically increased and the lens was thinner in the experimental group. (Although the proliferation of lens cells increased in the experiment group but that lens had delay in removing the proliferated and elongation cells with abnormal density in the lateral part of the lens in compare with control group.) I have no idea what the authors are stating here. Moreover, the opening of the eyelids was delayed in the off springs of the mothers who received morphine. Conclusions: This study showed that morphine consumption during pregnancy leads to defects in fetal visual system development, particularly in the lens, and eyelids.
**Objetivo:** El consumo de morfina durante el embarazo, además de inducir anomalías en la función del sistema nervioso materno, provocó defectos o retrasos en la formación y el desarrollo del sistema visual embrionario. En el presente estudio, se evaluaron los cambios en el desarrollo del cristalino en embriones expuestos *in utero* a la morfina. **Materiales y métodos:** Se aparearon ratas hembra Wistar (250-300 g) con ratas macho y la preñez se determinó mediante la observación de espermatozoides en el frotis vaginal. Este día se consideró como el día embrionario cero (E0). Posteriormente, las hembras se dividieron aleatoriamente en el grupo experimental y el grupo control. El grupo control recibió agua del grifo y el grupo experimental recibió morfina (0,05 mg/ml) en el agua de bebida. En el día embrionario 13 (E13), se recolectaron muestras de sangre del seno retroorbitario de todos los animales para la cuantificación de corticosterona plasmática. En el día embrionario 17 (E17), los animales fueron sacrificados mediante una sobredosis de cloroformo y los embriones se extrajeron quirúrgicamente. Los embriones se fijaron en formalina al 10 % durante 30 días. Cumplido este periodo, se retiró la cabeza de los embriones para el procesamiento tisular y la tinción con hematoxilina-eosina (H&E). Las muestras se evaluaron mediante microscopía óptica y el software MOTIC. **Resultados:** Nuestros datos indicaron que el nivel plasmático de corticosterona se incrementó drásticamente y el cristalino fue más delgado en el grupo experimental. (Aunque la proliferación de células del cristalino aumentó en el grupo experimental, dicho cristalino presentó un retraso en la eliminación de las células proliferadas y en elongación, con una densidad anormal en la porción lateral del cristalino en comparación con el grupo control.) No tengo idea de lo que los autores están afirmando aquí. Asimismo, la apertura de los párpados se retrasó en las crías de las madres que recibieron morfina. **Conclusiones:** Este estudio demostró que el consumo de morfina durante el embarazo provoca anomalías en el desarrollo del sistema visual fetal, particularmente en el cristalino y los párpados.
Reference Key
bahadoran2012basicoral Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Hossein Bahadoran;Fatemeh Rostamkhani;Hedayat Sahraei;Homeira Zardooz;Hassan Ghoshoni;Sara Sadeghi-Gharachdaghi;Elaheh Tekieh;Masoomeh Kazemi
Journal gastrointestinal cancer: targets and therapy
Year 2012
DOI
DOI not found
URL
http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-105&slc_lang=en&sid=1
Keywords
visual system lens morphine neuropsychiatry biological psychiatry

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