induction of subacute ruminal acidosis affects the ruminal microbiome and epithelium

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ID: 142417
2016
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Abstract
Subacute ruminal acidosis (SARA) negatively impacts the dairy industry by decreasing dry matter intake, milk production, profitability, and increasing culling rate and death loss. Six ruminally-cannulated, lactating Holstein cows were used in a replicated incomplete Latin square design to determine the effects of SARA induction on the ruminal microbiome and epithelium. Experimental periods were 10 d with d 1 - 3 for ad libitum intake of control diet, followed by 50% feed restriction on d 4, and ad libitum access on d 5 to the basal diet or the basal diet with an additional 10% of a 50:50 wheat/barley pellet. Based on subsequent ruminal pH, cows were grouped (SARA grouping; SG) as Non-SARA or SARA based on time < 5.6 pH (0 and 3.4 h, respectively). Ruminal samples were collected on d 1 and 6 of each period prior to feeding and separated into liquid and solid fractions. Microbial DNA was extracted for bacterial analysis using 16S rRNA gene paired-end sequencing on the MiSeq Illumina platform and quantitative PCR (qPCR). Ruminal epithelium biopsies were taken on d 1 and 6 before feeding. Quantitative RT-PCR was used to determine gene expression in rumen epithelium. Bray-Curtis similarity indicated samples within the liquid fraction separated by day and coincided with an increased relative abundance of genera Prevotella, Ruminococcus, Streptococcus, and Lactobacillus on d 6 (P < 0.06). Although Firmicutes was the predominant phyla in the solid fraction, a SG × day interaction (P < 0.01) indicated a decrease on d 6 for SARA cows. In contrast, phylum Bacteroidetes increased on d 6 (P < 0.01) for SARA cows driven by greater genera Prevotella and YRC22 (P < 0.01). Streptococcus bovis and Succinivibrio dextrinosolvens populations tended to increase on d 6 but were not affected by SG. In ruminal epithelium, CLDN1 and CLDN4 expression increased on d 6 (P < 0.03) 24 h after SARA induction and a tendency for a SG × day interaction (P < 0.10) was observed for CLDN4. Overall, results indicate more rapid adaptation to an induced bout of SARA in the solid fraction ruminal microbiome compared with ruminal epithelium.
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mccann2016frontiersinduction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Joshua C Mccann;Shaoyu eLuan;Felipe C. Cardoso;Hooman eDerakhshani;Ehsan eKhafipour;Ehsan eKhafipour;Juan J. Loor;Juan J. Loor
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2016
DOI
10.3389/fmicb.2016.00701
URL
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00701/full
Keywords
bacteria rumen microbiome

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