intranasal vaccination with an engineered influenza virus expressing the receptor binding subdomain of botulinum neurotoxin provides protective immunity against botulism and influenza

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ID: 142192
2015
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Abstract
Influenza virus is a negative, segmented RNA virus without DNA intermediate. This makes it safer as a vaccine delivery vector than most DNA viruses that have potential to integrate their genetic elements into host genomes. In this study, we developed a universal influenza viral vector, expressing the receptor binding subdomain of botulinum neurotoxin A (BoNT/A).We tested the growth characters of the engineered influenza virus in chicken eggs and Madin-Darby Canine Kidney epithelial cells (MDCK), and showed that it can be produced to a titer of 5×10E6 plaque forming unites/ml in chicken eggs and MDCK cells. Subsequently, mice intranasally vaccinated with the engineered influenza virus conferred protection against challenge with lethal doses of active BoNT/A toxin and influenza virus. Our results demonstrated the feasibility to develop a dual purpose nasal vaccine against both botulism and influenza.
Reference Key
eli2015frontiersintranasal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Junwei eLi, Diana eDiaz-Arévalo, Yanping eChen, Mingtao eZeng
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Journal sudebno-meditsinskaia ekspertiza
Year 2015
DOI
10.3389/fimmu.2015.00170
URL
http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00170/full
Keywords
influenza allergy recombinant influenza vectorimmunologic diseases

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