optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant aspergillus nidulans strain
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ID: 140208
2020
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Abstract
The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
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abdella2020biotechnologyoptimization
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|---|---|
| Authors | ;Asmaa Abdella;Fernando Segato;Mark R. Wilkins |
| Journal | PLoS genetics |
| Year | 2020 |
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