direct chronopotentiometric analysis of riboflavin using a glassy carbon vessel as the working electrode
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ID: 139970
2016
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Abstract
A new method for the determination of riboflavin (vitamin B2) was developed
based on chronopotentiometry with a glassy carbon process vessel
macroelectrode. The method optimisation included investigation of the most
important experimental parameters: type and concentration of the supporting
electrolyte, initial potential, reduction current, and the working electrode
surface area. The reduction signal of riboflavin appeared at about -0.12 V
vs. Ag/AgCl (3.5 mol/dm3 KCl) electrode in 0.025 mol/dm3 HCl as the
supporting electrolyte. A linear response was obtained in the the range of
0.05-4 mg/dm3. The limit of detection and limit of quantitation were 0.018
mg/dm3 and 0.054 mg/dm3, respectively. Due to the use of specific working
electrode, a significant enhancement of the method relative sensitivity of
about 10 times was achieved. The accuracy of the defined method was
confirmed by HPLC analyses. The developed method was successfully applied
for the quantitation of riboflavin in various pharmaceutical multivitamin
preparations. [Projekat Ministarstva nauke Republike Srbije, br. III 46009]
| Reference Key |
.2016actadirect
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|---|---|
| Authors | ;Brezo Tanja Ž.;Stojanović Zorica S.;Suturović Zvonimir J.;Kravić Snežana Ž.;Kos Jovana J.;Milanović Spasenija D.;Đurović Ana D. |
| Journal | chemical papers |
| Year | 2016 |
| DOI |
10.2298/APT1647143B
|
| URL | |
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