chronic low dose rate ionizing radiation exposure induces premature senescence in human fibroblasts that correlates with up regulation of proteins involved in protection against oxidative stress

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ID: 137936
2014
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Abstract
The risks of non-cancerous diseases associated with exposure to low doses of radiation are at present not validated by epidemiological data, and pose a great challenge to the scientific community of radiation protection research. Here, we show that premature senescence is induced in human fibroblasts when exposed to chronic low dose rate (LDR) exposure (5 or 15 mGy/h) of gamma rays from a 137Cs source. Using a proteomic approach we determined differentially expressed proteins in cells after chronic LDR radiation compared to control cells. We identified numerous proteins involved in protection against oxidative stress, suggesting that these pathways protect against premature senescence. In order to further study the role of oxidative stress for radiation induced premature senescence, we also used human fibroblasts, isolated from a patient with a congenital deficiency in glutathione synthetase (GS). We found that these GS deficient cells entered premature senescence after a significantly shorter time of chronic LDR exposure as compared to the GS proficient cells. In conclusion, we show that chronic LDR exposure induces premature senescence in human fibroblasts, and propose that a stress induced increase in reactive oxygen species (ROS) is mechanistically involved.
Reference Key
loseva2014proteomeschronic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Olga Loseva;Emman Shubbar;Siamak Haghdoost;Bastiaan Evers;Thomas Helleday;Mats Harms-Ringdahl
Journal north carolina medical journal
Year 2014
DOI
10.3390/proteomes2030341
URL
http://www.mdpi.com/2227-7382/2/3/341
Keywords
Proteomics ionizing radiation human fibroblastsmicrobiology

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