production of indian citrus ringspot virus free plants of kinnow employing chemotherapy coupled with shoot tip grafting

Clicks: 283
ID: 137860
2007
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Abstract
Indian citrus ringspot virus (ICRSV) is known to cause serious problem in Kinnow (Citrus nobilis Lour × C. deliciosa Tenora). This paper reports the elimination of ICRSV from Kinnow by chemotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect ELISA and RT-PCR) were cultured on MS medium containing 2-iP (1mg/l) and malt extract (800 mg/l) along with different concentrations of five antiviral chemicals acycloguanosine, azidothymidine, 2,4-dioxohexahydro-1, 2,5-triazine (DHT), ribavirin and 2- thiouracil. Shoot tips of size 0.7 mm were excised from the sprouts of these nodal segments and grafted on to rough lemon (Citrus jambhiri) under aseptic conditions. The plantlets obtained from chemotherapy coupled with in vitro micrografting were indexed by indirect ELISA and RT-PCR after acclimatization. Maximum effect (37% virus elimination) was seen for ribavirin at 25 mg/l followed by 2-thiouracil at 25 mg/l (21.4%) and acyclguanosine at 25 mg/l (20.8%). Azidothymidine and DHT at the tested doses could not eliminate ICRSV. In the present study only those plants/plantlets were considered virus free, which showed negative reaction both with indirect ELISA and RT-PCR.
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sanjeev2007journalproduction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Sharma SANJEEV;Singh BALWINDER;Rani GITA;Zaidi AIJAZ ASGHAR;Hallan VIPIN;Nagpal AVINASH;Virk GURDEEP SINGH
Journal balkan medical journal
Year 2007
DOI
DOI not found
URL
http://jcea.agr.hr/articles/395_PRODUCTION_OF_INDIAN_CITRUS_RINGSPOT_VIRUS_FREE_PLANTS_OF_KINNOW_EMPLOYING_CHEMOTHERAPY_COUPLED_WITH_SHOOT_TIP_GRAFTING_en.pdf
Keywords
Citrus shoot tip graftingagriculture

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