measurement of antioxidant activity towards superoxide in natural waters.

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ID: 133101
2016
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Abstract
Antioxidants are a class of molecules that provide a protective function against reactive oxygen species (ROS) in biological systems by out competing physiologically important molecules for ROS oxidation. In natural waters, the reactivity of antioxidants gives an estimate of oxidative stress and may determine the reactivity and distribution of reactive oxidants. We present an analytical method to measure antioxidant activity in natural waters through the competition between ascorbic acid, an antioxidant, and MCLA, a chemiluminescent probe for superoxide. A numerical kinetic model of the analytical method has been developed to optimize analytical performance. Measurements of antioxidant concentrations in pure and seawater are possible with detection limits below 0.1 nM. Surface seawater samples collected at solar noon contained over 0.4 nM of antioxidants and exhibited first-order decay with a half-life of 3-7 minutes, consistent with a reactive species capable of scavenging photochemically produced superoxide.
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king2016frontiersmeasurement Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;D. Whitney King;Emma Berger;Zachary Helm;Eleanor Irish;Kenneth Mopper
Journal journal of aquatic food product technology
Year 2016
DOI
10.3389/fmars.2016.00217
URL
http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00217/full
Keywords
xanthine oxidase superoxide geographical distribution including nature conservation

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