production of isoflavones, daidzein and genistein in callus cultures of pueraria candollei wall. ex benth. var. mirifica

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ID: 132631
2006
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Abstract
Callus cultures of Pueraria candollei var. mirifica were first established from various parts of explants with the objective of isoflavones, daidzein and genistein production. The cultures were studied on their growth and isoflavone production by various combinations of growth regulators, auxins and cytokinins at 25±2ºC. Daidzein and genistein accumulated in the cells were determined. The results revealed that callus of P. candollei var. mirifica was capable of producing high level of both isoflavones consistently. The culture temperature played an important role in the growth and isoflavone production. Over twofold of growth and threefold of isoflavone production were demonstrated at 32±2ºC. The callus established from the stems in MS medium supplemented with 4.5 µM 2,4-D and 0.46 µM kinetin produced the highest yield of daidzein (5.12 mg/g, DW) and genistein (2.77 mg/g, DW), which was remarkably higher than the intact plants.
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panichajakul2006songklanakarinproduction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Sanha Panichajakul;Sudarat Thanonkeo
Journal ferroelectrics
Year 2006
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