simple detection of phosphoproteins in sds-page by quercetin

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ID: 132377
2014
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Abstract
A novel fluorescence-based staining method was developed for phosphoprotein analysis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Similar to the mechanism of immobilized metal ion affinity chromatography (IMAC), the method employed quercetin–aluminum (III)-appended complex as a fluoroprobe to selectively visualize phosphorylated proteins among total proteins. According to the results, as low as 16–32 ng of phosphoproteins (α-casein, β-casein and phosvitin) could be selectively detected in 90 min with a wide linear dynamic range. In addition, the specificity of this novel stain for phosphoproteins was confirmed by 1-D and 2-D SDS-PAGE, dephosphorylation, western blot and liquid chromatography–mass spectrometry analysis (LC–MS/MS), respectively.
Reference Key
wang2014eupasimple Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xi Wang, Maowei Ni, Chao Niu, Xinliang Zhu, Ting Zhao, Zhongxin Zhu, Yuanhu Xuan, Weitao Cong
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Journal technological and economic development of economy
Year 2014
DOI
10.1016/j.euprot.2014.07.002
URL
http://www.sciencedirect.com/science/article/pii/S2212968514000476
Keywords
quercetin aluminum

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