simple detection of phosphoproteins in sds-page by quercetin
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ID: 132377
2014
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Abstract
A novel fluorescence-based staining method was developed for phosphoprotein analysis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Similar to the mechanism of immobilized metal ion affinity chromatography (IMAC), the method employed quercetin–aluminum (III)-appended complex as a fluoroprobe to selectively visualize phosphorylated proteins among total proteins. According to the results, as low as 16–32 ng of phosphoproteins (α-casein, β-casein and phosvitin) could be selectively detected in 90 min with a wide linear dynamic range. In addition, the specificity of this novel stain for phosphoproteins was confirmed by 1-D and 2-D SDS-PAGE, dephosphorylation, western blot and liquid chromatography–mass spectrometry analysis (LC–MS/MS), respectively.
| Reference Key |
wang2014eupasimple
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|---|---|
| Authors | ;Xi Wang;Maowei Ni;Chao Niu;Xinliang Zhu;Ting Zhao;Zhongxin Zhu;Yuanhu Xuan;Weitao Cong |
| Journal | technological and economic development of economy |
| Year | 2014 |
| DOI |
10.1016/j.euprot.2014.07.002
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