Colorimetric aflatoxins immunoassay by using silica nanoparticles decorated with gold nanoparticles.

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ID: 128071
2020
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Abstract
Nanomaterials-based colorimetric immunoassays showed increasing attention for monitoring different biomarkers because of their unique optical and electrical features. Here, a highly sensitive and selective optical sensor was described for the determination of different aflatoxins (AFs). Mesoporous silica nanoparticles (m-SNPs) with an average particle size of 40 nm were prepared by the sol-gel method and then decorated with gold nanoparticles (AuNPs). The Au NPs@m-SiNPs nanocomposite with an average particle size of 66 nm was modified with AFs antibodies. The assay includes the following steps: the Au NPs@m-SiNPs nanocomposite was immersed with AFs antibodies, and then the AFs-Ab/Au NPs@m-SiNPs was used as a probe for AFs detection. The interaction between the AFs-Ab/Au NPs@m-SiNPs and the AFs has resulted in a change in its color from pink to violet. Measurements are performed by absorptiometry at a wavelength of 425 nm. The immunoassay works in the concentration range from 1 ng·mL to 75 ng·mL AFB1 and has a limit of detection 0.16 ng·mL (at S/N = 3). The assay was applied to the determination of AFs in different food samples spiked with AFS. Finally, the assay was used to detect AFs in a real sample, and the LC-MS technique was used to verify the results.
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althagafi2020colorimetricspectrochimica Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Althagafi, Ismail I;Ahmed, Saleh A;El-Said, Waleed A;
Journal spectrochimica acta part a, molecular and biomolecular spectroscopy
Year 2020
DOI
S1386-1425(20)30978-1
URL
Keywords
mycotoxins food safety environmental monitoring optical sensor aflatoxin antibodies mesoporous silica@gold nanocomposite

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