Enzymatic modification of daidzin using heterologously expressed amylosucrase in .

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ID: 12115
2019
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Abstract
Amylosucrases (ASase, EC 2.4.1.4) from (DGAS) and (NPAS) were heterologously expressed in While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in . Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.
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kim2019enzymaticfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kim, Eun-Ryoung;Rha, Chan-Su;Jung, Young Sung;Choi, Jung-Min;Kim, Gi-Tae;Jung, Dong-Hyun;Kim, Tae-Jip;Seo, Dong-Ho;Kim, Dae-Ok;Park, Cheon-Seok;
Journal Food science and biotechnology
Year 2019
DOI
10.1007/s10068-018-0453-7
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