Polymerase chain reaction detection of nonviable bacterial pathogens

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ID: 120358
1993
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Abstract
Polymerase chain reaction (PCR) methodologies for detection of pathogens in environmental samples are currently available. However, positive amplification products for any set of primers only signal that the appropriate target nucleic acid sequences were present in the sample. The presence of the am …
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Authors Josephson KL;Gerba CP;Pepper IL;;
Journal Applied and environmental microbiology
Year 1993
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/8250575
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE pubmed abstract nih national institutes of health national library of medicine DNA Bacteria / isolation & purification* Polymerase Chain Reaction / methods* bacteria / pathogenicity escherichia coli / isolation & purification evaluation studies as topic microbial false positive reactions bacterial / genetics escherichia coli / genetics bacterial / isolation & purification colony count bacteria / genetics* pmid:8250575 pmc182487 doi:10.1128/aem.59.10.3513-3515.1993 k l josephson c p gerba i l pepper environmental microbiology salmonella typhi / genetics salmonella typhi / isolation & purification shigella sonnei / genetics shigella sonnei / isolation & purification

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