Expression of JC virus agnoprotein in progressive multifocal leukoencephalopathy brain

Clicks: 177
ID: 116460
2002
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #208 of 438 articles by views in acta neuropathologica

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 438 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
To examine the function of JC virus (JCV) agnoprotein, we examined the brains of cases of progressive multifocal leukoencephalopathy (PML), which is caused by JCV infection, using a newly generated antibody. The antibody reacted with 8 kDa protein specific for JCV agnoprotein by Western blotting. In vitro analyses showed that JCV capsid protein VP1 and large T antigen (T-Ag) were localized in the nuclei, but that agnoprotein was mainly detected in the cytoplasm of JCV-infected cells with an occasional nuclear staining. In the PML brain, an immunoreactive signal for agnoprotein was distributed in the perinuclear areas and cytoplasmic processes with occasional punctate staining in demyelinating lesions as well as adjacent myelinated areas. Agnoprotein presented mostly in the infected oligodendrocytes and partly in the astrocytes. Using double immunostaining, agnoprotein was seen to be expressed in the cytoplasmic processes of the cells, the nuclei of which were labeled with VP1 and T-Ag, where virus particles existed. Thus, JCV agnoprotein was mostly expressed in the infected oligodendrocytes and mainly localized in the cytoplasmic processes apart from virus particles in the demyelinated lesions.
Reference Key
okada2002actaexpression Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yuki Okada;Hirofumi Sawa;Shuichi Endo;Yasuko Orba;Takashi Umemura;Hiroshi Nishihara;Alexandru C. Stan;Shinya Tanaka;Hidehiro Takahashi;Kazuo Nagashima;Yuki Okada;Hirofumi Sawa;Shuichi Endo;Yasuko Orba;Takashi Umemura;Hiroshi Nishihara;Alexandru C. Stan;Shinya Tanaka;Hidehiro Takahashi;Kazuo Nagashima;
Journal acta neuropathologica
Year 2002
DOI
doi:10.1007/s00401-002-0526-8
URL
https://link.springer.com/article/10.1007/s00401-002-0526-8
Keywords
pathology neurosciences

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.