Amplification of cDNA ends based on template-switching effect and step-out PCR

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1999
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Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl …
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Authors Matz M;Shagin D;Bogdanova E;Britanova O;Lukyanov S;Diatchenko L;Chenchik A;;
Journal Nucleic Acids Research
Year 1999
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/10037822
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE genetic pubmed abstract nih national institutes of health national library of medicine models research support non-u.s. gov't DNA Polymerase Chain Reaction / methods* pmid:10037822 pmc148354 doi:10.1093/nar/27.6.1558 m matz d shagin a chenchik complementary / genetics*

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