Virulence plasmid stability in environmentally occurring Bacillus anthracis from North East Turkey

Clicks: 135
ID: 115892
2016
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #186 of 260 articles by views in antonie van leeuwenhoek

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 260 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The Bacillus anthracis virulence plasmid pXO2, which encodes for a polypeptide capsule, can be lost during long term laboratory storage. To determine if pXO2 is lost in nature we screened B. anthracis isolates obtained from B. anthracis spores from contaminated animal burial sites in Turkey for their ability to express a capsule upon primary culture. A total of 672 B. anthracis colonies were examined of which ten produced a mixed mucoid (capsule +ve)/non-mucoid (capsule −ve) phenotype and a further one colony yielded non-mucoid colonies upon repeated culture. Screening by PCR using pXO2 specific primers revealed that seven of these isolates had eliminated the plasmid. Of the four colonies which were positive by PCR, one regained the ability to express a capsule upon repeated culture suggesting that the defect was reversible. This is an important observation as capsule expression is a principal marker of virulence and in the absence of PCR serves as a key diagnostic marker. The results of this preliminary study suggest that pXO2 is lost in nature and that further studies are need to determine the mechanisms by which this occurs.
Reference Key
cooper2016antonievirulence Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Callum Cooper;Fatih Buyuk;Bettina Schelkle;Aliye Gulmez Saglam;Elif Celik;Ozgur Celebi;Mitat Sahin;Tom Hawkyard;Les Baillie;Callum Cooper;Fatih Buyuk;Bettina Schelkle;Aliye Gulmez Saglam;Elif Celik;Ozgur Celebi;Mitat Sahin;Tom Hawkyard;Les Baillie;
Journal antonie van leeuwenhoek
Year 2016
DOI
doi:10.1007/s10482-016-0767-5
URL
https://link.springer.com/article/10.1007/s10482-016-0767-5
Keywords
Microbiology plant sciences medical microbiology soil science & conservation

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.