Glial fibrillary acidic protein expression in a new human glioma cell line in culture before and after xenogenic transplantation into nude mice

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ID: 113905
1970
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Abstract
A human glioma cell line, SA146, was initiated on precoated extracellular matrix from a stereotactic biopsy of a glioblastoma. We report modulation in the expression of glial fibrillary acidic protein (GFAP) by SA146 passed in vitro before or after xenogenic transplantation into nude mice. Immunofluorescence data show a decrease in the percentage of GFAP-expressing cells with increasing in vitro passages but a full reexpression (100% of GFAP-positive cells among vimentin-positive cells) was observed in cultures just derived from the xenotransplanted tumor. These changes are correlated with the mRNA content (Northern blot probed with a cDNA for GFAP) and with the protein level (cytoskeletal fraction analyzed by two-dimensional gel electrophoresis and Western blots probed with a monoclonal antibody). At the optimal level of GFAP expression, a large range of microheterogeneity in GFAP isoforms is reached for which post-translational events are clearly involved since mRNA translation in cell free system would provide at best three isomers. We suggest that SA146 would be an appropriate model to study the regulation of GFAP expression in the context of human glial tumor biology.
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gomès1970actaglial Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors D. Gomès;B. de Néchaud;R. Maunoury;V. Moura Neto;C. Brigaudeau;F. Labrousse;P. Dupouey;D. Gomès;B. de Néchaud;R. Maunoury;V. Moura Neto;C. Brigaudeau;F. Labrousse;P. Dupouey;
Journal acta neuropathologica
Year 1970
DOI
doi:10.1007/s004010050722
URL
https://link.springer.com/article/10.1007/s004010050722
Keywords
pathology neurosciences

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