Performance of a Pneumolysin Enzyme-Linked Immunosorbent Assay for Diagnosis of Pneumococcal Infections

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ID: 113730
2007
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Abstract
A pneumolysin-specific enzyme-linked immunosorbent assay (PLY-ELISA) for the detection of pneumolysin in urine was developed and evaluated in comparison with the commercially available Binax Now Streptococcus pneumoniae test (Binax, Portland, ME) for the diagnosis of pneumococcal infections. Assay sensitivity was evaluated using urine from 108 patients with culture-confirmed pneumococcal infections. In adults, the sensitivity and specificity of the PLY-ELISA were 56.6% and 92.2%, respectively. In children with nasopharyngeal pneumococcal carriage, PLY-ELISA and Binax Now S. pneumoniae test sensitivities were 62.5% and 87.5%, respectively, while specificities were 94.4% and 27.8%, respectively. In children with nonnasopharyngeal pneumococcal carriage, PLY-ELISA and Binax Now S. pneumoniae test sensitivities were 68.7% and 93.7%, respectively, and test specificities were 94.1% and 41.2%, respectively. The persistence of pneumolysin in urine of pneumococcal pneumonia patients decreased significantly after 4 to 6 days of treatment. Our data suggest that combining the high specificity of the PLY-ELISA with the high sensitivity of the Binax Now S. pneumoniae test would enable pneumococcal infections to be accurately diagnosed in children.
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Authors María del Mar García-Suárez;María Dolores Cima-Cabal;Roberto Villaverde;Emma Espinosa;Miquel Falguera;Juan R. de Los Toyos;Fernando Vázquez;Francisco J. Méndez;María del Mar García-Suárez;María Dolores Cima-Cabal;Roberto Villaverde;Emma Espinosa;Miquel Falguera;Juan R. de Los Toyos;Fernando Vázquez;Francisco J. Méndez;
Journal Journal of clinical microbiology
Year 2007
DOI
10.1128/JCM.01030-07
URL
http://dx.doi.org/10.1128/JCM.01030-07
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't adult female male adolescent aged middle aged 80 and over sensitivity and specificity pneumococcal infections / diagnosis* maría dolores cima-cabal francisco j méndez streptolysins / urine* enzyme-linked immunosorbent assay / methods* pmid:17728474 pmc2168496 doi:10.1128/jcm.01030-07 maría del mar garcía-suárez bacterial proteins / urine pneumococcal infections / urine

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