Experimental discrimination and molecular characterization of the extracellular soil DNA fraction
Clicks: 210
ID: 113392
2009
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
210 views
50 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #82 of 260 articles by views in antonie van leeuwenhoek
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 260 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
We experimentally discriminated and quali-quantitatively characterized the extracellular fraction of a forest soil DNA pool. We sequentially extracted and classified the components of extracellular DNA by its strength of interaction with soil colloids as: (1) extractable in water, free in the extracellular soil environment or adsorbed on soil colloids; and as (2) extractable in alkaline buffer after previous extraction in water, bound on soil colloids. The comparative molecular analysis (fluorometer, gel electrophoresis, genetic fingerprinting) of directly and sequentially extracted extracellular DNA revealed quantitative and qualitative differences, also in terms of genetic information about microbial communities. The sequential extraction of extracellular DNA revealed differences in molecular weight, indicating a relationship between DNA fragment length and strength of interaction with soil colloids. The sequential extraction was also suitable to assess the presence of tightly bound DNA, providing information about the DNA-colloid interactions naturally occurring in the soil environment.
| Reference Key |
ceccherini2009antonieexperimental
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Maria Teresa Ceccherini;Judith Ascher;Alberto Agnelli;Federica Borgogni;Ottorino Luca Pantani;Giacomo Pietramellara;Maria Teresa Ceccherini;Judith Ascher;Alberto Agnelli;Federica Borgogni;Ottorino Luca Pantani;Giacomo Pietramellara; |
| Journal | antonie van leeuwenhoek |
| Year | 2009 |
| DOI |
doi:10.1007/s10482-009-9354-3
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.