Amplification of cDNA ends based on template-switching effect and step-out PCR.
Clicks: 108
ID: 112938
1999
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Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. ...
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| Reference Key |
chenchik1999nucleicamplification
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|---|---|
| Authors | M Matz, D Shagin, E Bogdanova, O Britanova, S Lukyanov, L Diatchenko, A Chenchik;M Matz;D Shagin;E Bogdanova;O Britanova;S Lukyanov;L Diatchenko;A Chenchik; |
| Journal | Nucleic Acids Research |
| Year | 1999 |
| DOI |
10.1093/nar/27.6.1558
|
| URL | |
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