Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA

Clicks: 345
ID: 112251
2007
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Abstract
The accurate diagnosis of pneumococcal disease has frequently been hampered not only by the difficulties in obtaining isolates of the organism from patient specimens but also by the misidentification of pneumococcus-like viridans group streptococci (P-LVS) as Streptococcus pneumoniae. This is especi …
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Authors Carvalho Mda G;Tondella ML;McCaustland K;Weidlich L;McGee L;Mayer LW;Steigerwalt A;Whaley M;Facklam RR;Fields B;Carlone G;Ades EW;Dagan R;Sampson JS;;
Journal Journal of clinical microbiology
Year 2007
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/17537936
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't u.s. gov't non-p.h.s. Child DNA Genes sensitivity and specificity Evaluation Study Polymerase Chain Reaction / methods* bacterial* cerebrospinal fluid / microbiology streptococcus pneumoniae / genetics streptococcus pneumoniae / isolation & purification* false positive reactions pneumococcal infections / diagnosis bacterial / analysis* bacterial / genetics nucleic acid hybridization pmid:17537936 pmc1951257 doi:10.1128/jcm.02498-06 maria da gloria s carvalho maria lucia tondella jacquelyn s sampson otitis media with effusion / microbiology pneumococcal infections / microbiology* serum / microbiology

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